ABSTRACT
Medicinal plants are valuable natural resources that are nowadays known as safe pre-materials for drug industries in the advanced countries. Black caraway is one of the most important and economical medicinal plants that can be grown naturally in some regions of Yazd province. The essence of the plant are used in treatment of diarrhea, dyspepsia, fever, flatulence, stomachache, hemorrhoids, hiccoughs, as an antihistaminic and more numerous uses in medicine industries. This plant shows several therapeutic effects on digestive and urinary tract disorders and is well known as an anti-convulsant, anthelmintic, anti-asthma and antidyspnic drug in Iranian traditional medicine. Bunium Persicum oil is capable of suppressing the initial stage of an inflammatory process. Also, this plant is used for culinary purposes and for flavoring foods and beverages. In this research, the fractionization of seed essence was done by GC method and the compounds were recognized. The DPPH test was used for estimating anti oxidant effects and the Follin-Ciocalteu method was used for estimating quantity of phenol compounds. The results revealed that- gammaTerpinene had the highest percentage in the essence of the native black caraway of Yazd province. Also, the antioxidant test showed the high antioxidant effect of the native black caraway of Yazd province with an IC50 of 2.85 microg.mg-1 and the phenolic component percentage of 117.09mg.g1. This result was much higher than previous similar studies about black caraway
Subject(s)
Monoterpenes , Terpenes , Antioxidants , Plant ExtractsABSTRACT
Narcissus tazetta of the Amaryllidaceae is a monocotyledon, perennial and bulbous plant. This ornamental fragrant plant has various alkaloids that have shown anti tumor, anti virus and anticholinergic activities. In this study, we have investigated the alkaloids of the bulbs of Narcissus tazetta [post flowering] from Gilan province, north of Iran. Ethanolic extract of bulbs were prepared. Then the alkaloids were extracted with different solvents [Et[2]O, CHCI[3], Na[2]CO[3] solution] to yielding a brown gum. This extract was Done chromatography by TLC on silica gel. The separated alkaloids were identified by the study of their NMR, IR and MASS spectra. The bulbs of Narcissus tazetta from Gilan province were found to contain homolycorine as the major alkaloid as well as tazettine and ismine. This is the first report of ismine identification in the N. tazetta whereas tazettine and demethylated derivative of homolycorine, that have medicinal properties, were found previously
ABSTRACT
The purpose of this study was to achieve defense proteins. Indeed, it proposed that salicylic acid [SA] involved a number of defense mechanisms. The influence of SA application probably on changes in levels of protein extent of Dianthus shoots was investigated. Firstly, explants of cv. Cerise Royallette were cultured on OM medium with 0.2 mg. L-1 NAA, 0.5 mg. L-1 BAP, supplemented by 0, 10, 50, and 200 microM SA. After 4 weeks, growth of shoots were measured and then prepared extracts and performed Bradford test. These substances were tested with SDS-PAGE, to find the concentrations which caused changes in quality of proteins. Data analysis indicated that the most typical effect of SA treatment protein content of shoots was occurred. Indeed, treatment increased protein content considerably, however, it decreased the number of shoots. Analysis of the polypeptide pattern following SDS-PAGE showed that all tested SA changed the accumulation of both 38 and 39 kDa polypeptides that located very close to each others, and the level of their accumulation was found to be varied depending on the kind and intensity of the stressor. The data provide evidences that plant defense mechanisms might involve the same proteins in response to SA and may lead to induction or deletion of defense proteins changes